Further Studies on the Oxidation of Extramitochondrial Reduced Nicotineamide-Adenine Dinucleotide in Landschuetz Ascites Tumor Cells
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چکیده
منابع مشابه
The oxidation of extramitochondrial reduced diphosphopyridine nucleotide in ascites tumor cells.
The capability of oxaloacetate and a-ketoglutarate to replace pyruvate in its interconversion with lactate in the presence of Landschuetz ascites tumor cells was investigated. Using lactate1-14C,it was shown that oxaloacetate brought about a considera ble accumulation of pyruvate-l-I4C and stimulated both 02 con sumption and 14C02production. Oxaloacetate also enhanced the removal of lactate and...
متن کاملThe Oxidation of Extramitochondrial Reduced Diphosphopyridine Nucleotide in Ascites Tumor Cells1
The capability of oxaloacetate and a-ketoglutarate to replace pyruvate in its interconversion with lactate in the presence of Landschuetz ascites tumor cells was investigated. Using lactate1-14C,it was shown that oxaloacetate brought about a considera ble accumulation of pyruvate-l-I4C and stimulated both 02 con sumption and 14C02production. Oxaloacetate also enhanced the removal of lactate and...
متن کاملNicotinamide Adenine Dinucleotide Synthesis in Ascites Cells in Vitro.
Previous studies in our laboratory (1) have shown that intact ascites cells incorporate nicotinamide-14C into both nicotinamide adenine dinucleotide and nicotinic acid adenine dinucleotide. It was also shown that the specific activity of the nicotinamide adenine dinucleotide increased with increasing concentrations of nicotinamide-14C. High levels of nicotinic acid-r4C, on the other hand, did n...
متن کاملStudies on the Respiratory Chain-linked Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase XI. TRANSFORMATION OF THE DEHYDROGENASE TO REDUCED NICOTINAMIDE ADENINE
Purified preparations of the reduced nicotinamide adenine dinucleotide dehydrogenase of the respiratory chain, extracted with the aid of phospholipase at moderate temperature, do not catalyze the reduction of long chain coenzyme Q derivatives (Q6 and QIO) at significant rates and are not inhibited by amytal or rotenone. On exposure of the enzyme to conditions which have been used for the extrac...
متن کاملStudies on the respiratory chain-linked reduced nicotinamide adenine dinucleotide dehydrogenase. XI. Transformation of the dehydrogenase to reduced nicotinamide adenine dinucleotide-coenzyme Q reductase.
Purified preparations of the reduced nicotinamide adenine dinucleotide dehydrogenase of the respiratory chain, extracted with the aid of phospholipase at moderate temperature, do not catalyze the reduction of long chain coenzyme Q derivatives (Q6 and QIO) at significant rates and are not inhibited by amytal or rotenone. On exposure of the enzyme to conditions which have been used for the extrac...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1968
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1968.tb00331.x